TC1細(xì)胞

細(xì)胞形態(tài)： **樣

**類型： mouse IgG1 kappa , IgG1; kappa light chain

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CRL-2493?

Designations: TC1

TC1細(xì)胞Depositors: DG Sieckmann

Isotype: mouse IgG1 kappa , IgG1; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybrid

Cellular Products: immunoglobulin; monoclonal antibody; against an idiotypic determinant on TC-31 monoclonal antibody (against exotoxin A)

Permits/Forms: TC1細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: This cell line was deposited at the ATCC by Donna G. Sieckmann, Naval Medical Research Center. The line is distributed for academic research purposes only. The Naval Medical Research Center releases the line subject to the following: 1.) The line or its products must not be distributed to third parties. Commercial interests are the exclusive property of the Naval Medical Research Center. 2.) Any proposed commercial use of the cell line including any use by a for-profit entity must first be negotiated with the Commanding Officer, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910-7500.

Isolation: Isolation date: 1984

Comments: Animals were immunized with purified exotoxin A of Pseudomonas aeruginosa.

Spleen cells were fused with Sp2/0-Ag14 cells.

The antibody binds specifically for the exotoxin A of Pseudomonas aeruginosa.

It displays specificity for SDS-denatured exotoxin A from the strain PA103 and its derivative PA103/29 but does not react with exotoxin from strain PAO1 or its derivative PAO-PR1. TC1細(xì)胞[40007]

The antibody shows high specificity and reactivity for denatured exotoxin A in immunoblotting and ELISA assays.

Both TC1 (ATCC CRL-2493) and TC31 (ATCC CRL-2494) antibodies neutralize exotoxin A toxicity against CHO cells in an in vitro assay but only TC31 protected mice infected with strain PA220 in an in vivo model. [40007]

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(4) viable cells/ml. Do not allow the cell concentration to exceed 5 X 10(5) viable cells/ml.

Interval: Maintain cultures at a cell concentration between 5 x 10(4) and 5 X 10(5) cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium TC1細(xì)胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020

References: 40007: Galloway DR, et al. Production and characterization of monoclonal antibodies to exotoxin A from Pseudomonas aeruginosa. Infect. Immun. 44: 262-267, 1984. PubMed: 6425217