SW 872細(xì)胞

數(shù)量： 大量

組織來(lái)源： connective tissue

細(xì)胞形態(tài)： 成纖維樣

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

ATCC Number： HTB-92?

相關(guān)**： 其他**

年限： 36 years *****

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

運(yùn)輸方式： 凍存運(yùn)輸

Designations: SW 872 [SW-872, SW872]

Depositors: A Leibovitz

SW 872細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Tissue: connective tissue

Disease: liposarcoma

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Isolation: Isolation date: 1974

Tumorigenic: Yes

Antigen Expression: Blood type O+

Cytogenetic Analysis: SW 872細(xì)胞hypertriploid; modal number = 80; range = 66 to 81. The rate of higher ploidies was 8.2%.Ten markers were common to most cells. These were: der(5)t(5;?)(q31;?)1, der(5)t(5;?)(q31;?)2, der(6)t(6;?)(q15;?), der(7)t(7;?)(q36;?), t(15q16q) and five others. Both der(5) markers, the der(7) and t(15q16q) were paired.There were 5 copies of N20 and N21, 4 copies of N8, N9, N11, N14 and N17 and a single copy of X in each cell.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

PGM1, 1-2

PGM3, 1

Age: 36 years *****

Gender: male

Ethnicity: Caucasian

Comments: The SW 872 cell line was initiated by A. Leibovitz in 1974 at the Scott and White Clinic, Temple, Texas from a surgical specimen of a fibrosarcoma removed from a 36 year old male Caucasian.

The histopathology evaluation reported an undifferentiated malignant tumor consistent with liposarcoma.

An ampule at passage 4 was received at the ATCC in January, 1982.

Propagation: SW 872細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 100%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: SW 872細(xì)胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008

recommended serum:ATCC 30-2020

References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

32289: Hu M, et al. Purification and characterization of human lung fibroblast motility-stimulating factor for human soft tissue sarcoma cells: identification as an NH2-terminal fragment of human fibronectin. Cancer Res. 57: 3577-3584, 1997. PubMed: 9270031