TT細胞

數量： 大量

生長狀態： 貼壁生長

是否是腫瘤細胞： 1

物種來源： 人

器官來源： 甲狀腺

細胞形態： 上皮樣

運輸方式： 凍存運輸

年限： 77 years

ATCC Number： CRL-1803?

相關**： 腫瘤

組織來源： medulla

TT細胞Designations: TT

Depositors: SS Leong

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: thyroid

Tissue: medulla

Disease: carcinoma

Cellular Products: calcitonin; carcinoembryonic antigen (CEA) [21867]

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. TT細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,13

D13S317: 11

D16S539: 12,13

D5S818: 12,13

D7S820: 10,12

THO1: 6,9

TPOX: 8,11

vWA: 16,18

Cytogenetic Analysis: aneuploid with several markers; modal number = 40; range = 32 to 86 [21867]

Age: 77 years

Gender: female

Ethnicity: Caucasian

Comments: TT細胞The TT cell line was established by S.S. Leong, et al. from a specimen obtained by needle biopsy from a 77 year old female with thyroid medullary carcinoma. [21867]

TT cells continuously produce high levels of calcitonin and CEA. [21867]

Immunoreactive calcitonin was found to be produced in cell culture at levels of 3900 pg/million cells and 7700 pg/million cells 24 and 72 hours respectively after a medium change. [21867]

CEA was found to accumulate to greater than 27 ng/million cells over a 72 hours period. [21867]

Chromosomal analysis of the cell line and tumors induced in nude mice reveal an aneuploid human karyotype with several marker chromosomes. [21867]

The initial characterization studies of the TT cell line were conducted using early passage TT cells cultivated in RPMI 1640 medium supplemented with 15% fetal bovine serum and 1 mM L-glutamine.

It is not known if the neuropeptides reported to be produced by this cell line when it was grown in RPMI 1640 medium are also produced by the cells when they are cultured in Ham's F12K medium.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended

Medium Renewal: Twice per week

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: Culture medium, 95%; DMSO, 5%

Doubling Time: 83 hrs

Related Products: TT細胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020

References: 21867: . Advances in thyroid neoplasia, 1981. Rome: Field Educational Italia; 1981.

32264: Behr TM, et al. Improved treatment of medullary thyroid cancer in a nude mouse model by combined radioimmunochemotherapy: doxorubicin potentiates the therapeutic efficacy of radiolabeled antibodies in a radioresistant tumor type. Cancer Res. 57: 5309-5319, 1997. PubMed: 9393755