PA-1細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

年限： 12 years

數(shù)量： 大量

是否是腫瘤細(xì)胞： 1

物種來源： 人

ATCC Number： CRL-1572?

相關(guān)**： 其他**

細(xì)胞形態(tài)： 上皮樣

生長狀態(tài)： 貼壁生長

器官來源： 卵巢

Designations: PA-1

PA-1細(xì)胞Depositors: BC Giovanella

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: ovary

Disease: teratocarcinoma

Derived from metastatic site: ascites

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: The cells form tightly knit colonies, and differentiate to form embryoid bodies when cultured in low serum concentration, or at low plating densities or when treated with 5-bromo-2'-deoxyuridine.

The embryonic antigen PCC4 is expressed, but F9 is not detectable.

Tumorigenic: PA-1細(xì)胞Yes

Oncogene: N-ras + (activated)

Antigen Expression: HLA A28, B12

DNA Profile (STR): Amelogenin: X

CSF1PO: 9,12

D13S317: 9,10

D16S539: 9,12

D5S818: 11

D7S820: 9

THO1: 7,9

TPOX: 11

vWA: 15,17

Isoenzymes: G6PD, B

Age: 12 years

Gender: female

Ethnicity: Caucasian

Comments: PA-1細(xì)胞The line was established from cells taken from ascitic fluid.

The cells form tightly knit colonies, and differentiate to form embryoid bodies when cultured in low serum concentration, or at low plating densities or when treated with 5-bromo-2'-deoxyuridine.

The embryonic antigen PCC4 is expressed, but F9 is not detectable.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

References: 22179: . . In Vitro 10: 382, 1974.

22368: Zeuthen J, et al. Characterization of a human ovarian teratocarcinoma-derived cell line. Int. J. Cancer 25: 19-32, 1980. PubMed: 6931103

22530: Giovanella BC, et al. Heterotransplantation of human malignant tumors in "nude" thymusless mice. II. Malignant tumors induced by injection of cell cultures derived from human solid tumors. J. Natl. Cancer Inst. 52: 921-930, 1974. PubMed: 4524119

22950: Tainsky MA, et al. PA-1細(xì)胞An activated rasN gene: detected in late but not early passage human PA1 teratocarcinoma cells. Science 225: 643-645, 1984. PubMed: 6740333

23112: McGowan-Jordan IJ, et al. Suppression of tumorigenicity in human teratocarcinoma cell line PA-1 by introduction of chromosome 4. Cancer Res. 54: 2568-2572, 1994. PubMed: 8168081