Hs 899(D)細(xì)胞
年限： 20 years
器官來(lái)源： 骨
數(shù)量： 大量
ATCC Number： CRL-7645?
相關(guān)**： 骨肉瘤
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
運(yùn)輸方式： 瓶裝運(yùn)輸
細(xì)胞形態(tài)： 成纖維樣
Hs 899(D)細(xì)胞生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
Designations: Hs 899(D).T
Biosafety Level: 1
Shipped: flask
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: bone
Disease: osteosarcoma
Derived from metastatic site: lung
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Hs 899(D)細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 20 years
Gender: male
Ethnicity: Caucasian
Comments: See also ATCC CCL-211 and ATCC CRL-7517, 7622, 7623, 7624, 7631, 7632, 7633, 7634, 7635, 7642, 7643 and 7644 for lines derived from the same patient.
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Please see the NBL Repository description.
Note: This item is distributed only within the 50 United States. It is not available for international distribution.
Propagation: Hs 899(D)細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:Hs 899(D)細(xì)胞ATCC 30-2020
